The Sample Processing Control (SPC) is spiked into samples before extraction to monitor poor extraction yield, PCR inhibition, incorrect pipetting or cycling parameters.
Positive controls
Positive controls allow you to detect inhibited amplification reactions due to the carryover of reagents used for the isolation of nucleic acids. Other causes of false-negative results include target nucleic acid degradation, sample processing errors and thermocycler malfunction.
Benefits :
- Specific: Does not interfere with target amplification and avoid amplification of endogenous genes
- Sensitive: Compatible with low copy templates and available with dark quencher for maximal signal-to-noise ratio
- Designed for multiplex assays: Detected in the Yakima Yellow®/VIC®/and JOE channel. Validated in duplex assays with the target in the FAM channel
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